Coding
AID-T7 RNA

Part:BBa_K5005003:Design

Designed by: YunQi Chen   Group: iGEM23_GEC-CHINA   (2023-10-05)


Expresses editor under dox induction.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1088
    Illegal XhoI site found at 1822
    Illegal XhoI site found at 2119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2598
    Illegal AgeI site found at 3322
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1308
    Illegal BsaI site found at 2620
    Illegal BsaI.rc site found at 76
    Illegal BsaI.rc site found at 1094
    Illegal BsaI.rc site found at 3319
    Illegal SapI site found at 177
    Illegal SapI.rc site found at 1129
    Illegal SapI.rc site found at 1702
    Illegal SapI.rc site found at 2727


Design Notes

It is the key part of pEditor plasmid named AID-T7 RNAP-AID. It can express editor under doxycycline induction, which can edit genes downstream of the T7 promoter on the pTarget plasmids.

Source

Gifted by Professor Yu.

References

Chen, H., Liu, S., Padula, S., Lesman, D., Griswold, K., Lin, A., Zhao, T., Marshall, J. L., & Chen, F. (2020). Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor. Nature biotechnology, 38(2), 165–168. https://doi.org/10.1038/s41587-019-0331-8